Corn stover oil，as a kind of bio-oil，is low-carbon and renewable.At the same time，it may restore the performance of aged asphalt.In order to further promote the application of corn stover oil in road engineering，this study explored the effect of corn stover oil as a kind of regeneration agent on the rheological properties of aged asphalt through laboratory experiments and analyzed the regeneration effect.On this basis，the regeneration mechanism of corn stover oil regeneration agent was revealed by using an infrared spectroscopy test and gel permeation chromatography test.The results show that the addition of corn stover oil changes the proportion of aged asphalt components and restores the stability of the asphalt ’s colloidal structure to a certain extent，and it can dilute and blend aged asphalt to restore the physical and rheological properties of aged asphalt.However，the regeneration effect is affected by the addition amount of corn stover oil.When the addition amount of corn stover oil is 20%，it has the most significant effect in restoring aged asphalt performance.