To rejuvenate the excellent performance of aged styrene-butadiene-styrene （SBS） modified asphalt，colza oil bio-asphalt （COA） and polymethylene polyphenyl isocyanate （PAPI） were used in this study for adjusting the asphalt components and repairing SBS molecular structure，respectively.The above-mentioned dual regeneration mechanism was verified by comparing the effects of different rejuvenation components on the physical and rheological properties，as well as the molecular weight distribution of short-term aged SBS modified asphalt.Moreover，according to the change law of rheological properties of unaged base asphalt and SBS modified asphalt under the effect of dual regeneration agent，the influence of the regeneration agent on the phase angle platform area was excluded.Under the comprehensive consideration of the physical and rheological properties of dual regeneration SBS modified asphalt，the optimal contents of COA and PAPI were determined.The experimental results indicate that the double regeneration agent can not only regulate the asphalt components but also connect the SBS molecular structure，restoring the physical and rheological properties of aged SBS modified asphalt.The optimal ratio of the dual regeneration agent is determined to be 12% COA and 1.25% PAPI.In addition，the fluorescence microscopy test results further verify that PAPI can rebuild the SBS network structure.